Design CRISPR guides with confidence
CRISPR guide design platform. SpCas9, SaCas9 and Cas12a are commercially available, with paired-site off-target analysis for SpCas9. Cas13d is implemented but disabled until its model licence is resolved.
New in the app
Lab protocol generator: from validated guide to oligo order in one click
Includes pX330/pX458 cloning oligos, PCR/Sanger primers, and expected amplicon size.
3 commercially enabled nucleases
SpCas9, SaCas9 and Cas12a are available; Cas13d remains a research implementation pending a commercial licence. For Cas12a the score is a relative-activity percentile among guides for the same target, not an editing percentage.
SpCas9
PAM: NGG
SaCas9
PAM: NNGRRT
Cas12a
PAM: TTTV
Everything you need in one tool
From sequence to optimal experimental design in under one second.
Efficiency prediction
SpCas9 XGBoost trained on DeepHF (Wang 2019, CC BY; over 55,000 guides). Pearson r = 0.8499 on its own hold-out.
Off-target risk
SpCas9 guide-site pair scoring (CFD, seed, mismatches, PAM) plus real genome-wide search with Cas-OFFinder. It does not replace experimental validation.
Multi-nuclease
SpCas9, SaCas9 and Cas12a are available, all three trained on commercially licensed data. Cas13d remains disabled while its licence is negotiated.
Base editing (CBE/ABE)
Predict efficiency and bystander risk for C→T and A→G base editors. Position-by-position activity window analysis.
Gene → Guides
Type BRCA1 or TP53 and get the best guides instantly from Ensembl or NCBI — no FASTA download required.
REST API + Stripe billing
Integrate via API keys. Upgrade to Basic or Advanced directly from the app with secure Stripe payments.
11 integrated analysis modules
Everything in one interface. No need to switch tools.
Sequence prediction
Analyse one or more guides (20 nt): efficiency, off-target, CrisprScore and molecular features.
Batch prediction (CSV)
Upload a CSV with hundreds of guides and get predictions for all of them in seconds.
PAM / FASTA scan
Upload a FASTA and get candidate guides with on-target efficiency; off-target scoring is available for SpCas9.
Compare guides
Visualise and compare multiple guides side by side with interactive charts.
Format CSV
Clean and normalise CSV files from any format to prepare them for prediction.
Experimental validation
Compare predictions against your own experimental data and calculate performance metrics (Pearson, Spearman, AUC).
Prediction history
Access all your previous predictions and download them at any time.
Multi-nuclease scan
Scan a FASTA with SpCas9, SaCas9 or Cas12a and filter by on-target activity. Cas13d will be enabled once its licence is resolved.
Base editing (CBE / ABE)
Predict efficiency and bystander risk for C→T and A→G editors with position-by-position window analysis.
Gene → Guides auto
Type BRCA1 or TP53 and get the best guides from Ensembl or NCBI without downloading any file.
Lab protocol generator
Generate cloning oligos (pX330/pX458), PCR/Sanger primers, and expected amplicon size to move from design to bench in one click.
Say it in your own words and the form fills itself
A conversational assistant inside the app. Describe what you need and it opens the right tab with the fields already set. It understands English and Spanish.
Your data never leaves the server
No external APIs, no third-party calls: all processing happens on the installation running the application — including the language model on deployments that ship it.
It proposes, it doesn't execute
It sets the form up and stops there. You review the values and press the button — nothing it says ever launches an analysis or spends quota.
It takes your files
Drop a FASTA or a CSV into the chat and it places it in the right tab. The file stays in your browser until you run it.
It never invents sequences
Any guide in a field is one you typed. Ask for an example and it uses published guides, telling you that's what they are.
Performance validated on independent datasets
SpCas9 evaluated on data never seen during training. Competitive with state-of-the-art published tools.
How it works
Choose your input
Paste a 20 nt sequence, upload a CSV, type a gene name (BRCA1, TP53) or upload a FASTA. The tool adapts to your workflow.
Choose nuclease and editor
SpCas9 by default. Switch to SaCas9 or Cas12a. Enable base editing (CBE/ABE) for single-nucleotide changes without DNA breaks.
Get results and export
Review activity, position and strand; compatible SpCas9 flows also include off-target scoring and CrisprScore. Sort and export to CSV.
Tell us what you need from a CRISPR tool
If your team needs a feature, nuclease or integration that isn't here, describe your case. We'll review it and reach out to understand it better. It's not a feature promise: it's the start of a conversation.
Start for free today
The Free plan includes 50 predictions per month, no credit card required. Scale up with Basic (€49/mo) or Advanced (€149/team).